PCR-based engineering of the yeast genome is an approach being used in my lab to study any visible intracellular structures (foci/filaments) formed by metabolic enzymes. The dynamically reversible assembly of several metabolic enzymes has been studied and characterized as a biological means to control enzymatic activity and pathway flux, in response to metabolic changes, starvation, and other stresses. Moreover, some of these enzymes might have some unprecedented moonlighting functions, in addition to their catalytic activity, that await to be revealed.

1. Nasalingkhan C, Sirinonthanawech N, Sato BK, Wilhelm JE, Noree C. Localization and regulation of yeast aldehyde dehydrogenase Ald4p structures. Heliyon. 2024 Oct 9;10(20):e39048.

2. Nasalingkhan C, Sirinonthanawech N, Noree C. Robust assembly of the aldehyde dehydrogenase Ald4p in Saccharomyces cerevisiae. Biology Open. 2023 Oct 15;12(10):bio060070.

3. Surasiang T, Noree C. Effects of A6E mutation on protein expression and supramolecular assembly of yeast asparagine synthetase. Biology. 2021;10(4):294.

4. Noree C, Sirinonthanawech N. Nuclear targeted Saccharomyces cerevisiae asparagine synthetases associate with the mitotic spindle regardless of their enzymatic activity. PLoS One. 2020 Dec 21;15(12):e0243742.

5. Noree C, Sirinonthanawech N. Coupled regulations of enzymatic activity and structure formation of aldehyde dehydrogenase Ald4p. Biology Open. 2020 Apr 15;9(4).

6. Noree C, Begovich K, Samilo D, Broyer R, Monfort E, Wilhelm JE. A quantitative screen for metabolic enzyme structures reveals patterns of assembly across the yeast metabolic network. Molecular Biology of the Cell. 2019 Oct 1;30(21):2721-36.

7. Noree C, Sirinonthanawech N, Wilhelm JE. Saccharomyces cerevisiae ASN1 and ASN2 are asparagine synthetase paralogs that have diverged in their ability to polymerize in response to nutrient stress. Scientific Reports. 2019 Jan 22;9(1):1-8.

8. Noree C, Monfort E, Shotelersuk V. Human asparagine synthetase associates with the mitotic spindle. Biology Open. 2018 Dec 15;7(12).

9. Noree C. Extramitochondrial assembly of mitochondrial targeting signal disrupted mitochondrial enzyme aldehyde dehydrogenase. Scientific Reports. 2018 Apr 18;8(1):1-6.